Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

Product Specs
ItemCell Counting Kit 8 (WST-8 / CCK8)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228554
Quantity1000 test
Sample TypeAdherent cells, Suspension cells
ApplicationsCellular Assay
TargetCell viability/proliferation

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Targeting of RRM2 suppresses DNA damage response and activates apoptosis in atypical teratoid rhabdoid tumorJ Exp Clin Cancer ResDecember 20, 2023Le Hien Giang, Kuo-Sheng Wu, Wei-Chung Lee, Shing-Shung Chu, Anh Duy Do, Chun A. Changou, Huy Minh Tran, Tsung-Han Hsieh, Hsin-Hung Chen, Chia-Ling Hsieh, et al.well, 6 replicate wells per plate. Cell growth inhibition assay was assessed with CCK8 kit (Abcam, UK, ab228554) by a microplate reader after 48 h of exposure to a serial dilution of COH29 (0.5–100 µM). The half-maximal

(2) Gypenoside IX restores Akt/GSK-3β pathway and alleviates Alzheimer's disease-like neuropathology and cognitive deficitsAging (Albany NY)December 12, 2023Ling Lei, Yong Luo, Dongkun Kang, Fumin Yang, Dongli Meng, Jian-Zhi Wang, Rong Liu, Xiaochuan Wang, Hong-Lian Litreated with different concentrations of STP without or with GP for different time. 10 μL CCK-8 reagent (ab228554, Abcam, Cambridge, MA, USA) was added into 90 μL DMEM to make the working solution. 100 μL working solution

(3) Improvement of Intracellular Interactions through Liquid Crystalline Elastomer Scaffolds by the Alteration of TopologyACS OmegaDecember 12, 2023Mahshid Fallah-Darrehchi, Payam Zahedigroups. At 0, 24, 48, and 72 h, the cell proliferation activity was detected using the CCK8 assay [Abcam (ab228554), MA]. In brief, the medium was replaced with fresh medium containing 10% CCK8 solution, and the plate

(4) Suppression of NK Cell Activation by JAK3 Inhibition: Implication in the Treatment of Autoimmune DiseasesJ Immunol ResDecember 8, 2023Wai Chung Wu, Carol Shiu, Tak Keung Tong, Shui On Leung, Chin Wai Huieach 96-well plate before treatment. After 3-day cytokine and drug incubation, 10 μl WST-8 reagent (ab228554, Abcam, Cambridge, UK) was added to each well and optical density of each well was measured at 450 nm

(5) Hydroxycitric Acid Alleviated Lung Ischemia-Reperfusion Injury by Inhibiting Oxidative Stress and Ferroptosis through the Hif-1α PathwayCurr Issues Mol BiolDecember 8, 2023Zi-Long Lu, Cong-Kuan Song, Shi-Shi Zou, Shi-Ze Pan, Kai Lai, Ning Li, Qing Gengplates for 12 h and then cultured with HCA at the corresponding dose for 24 h. The CCK8 assay kit (#ab228554, Abcam) was used to detect cell viability. HUVECs were cultured in 96-well plates; after appropriate

(6) Biocompatible Nanostructured Silver-Incorporated Implant Surfaces Show Effective Antibacterial, Osteogenic, and Anti-Inflammatory Effects in vitro and in Rat ModelInt J NanomedicineDecember 6, 2023Hui Gao, Nan Jiang, Qiannan Niu, Shenglin Mei, Håvard Jostein Haugen, Qianli Madesignated time points, the media were changed to 500 μL fresh CCK8 medium (GM or OM with 10% CCK-8, ab228554, Abcam, Netherlands), and the cells were cultured for an additional 2 h. The CCK8 medium was transferred

(7) miR-29c-3p represses the angiogenesis of esophageal squamous cell carcinoma by targeting SERPINH1 to regulate the Wnt signaling pathwayActa Cir BrasDecember 4, 2023Desheng Wei, Zhifeng Ma, Ting Zhu, Haiyong Wang, Bin Wang, Linhai Fu, Guangmao Yusubsequent experiments. CCK-8 assay Cell proliferation assay was performed on the cells using CCK-8 (ab228554, Abcam, United Kingdom). We seeded ESCC cells on 96-well plates with each well per 2×103 cells. Cells

(8) Modelling the disease: H2S-sensitivity and drug-resistance of triple negative breast cancer cells can be modulated by embedding in isotropic micro-environmentMaterials Today BioDecember 1, 2023Silvia Buonvino, Ilaria Arciero, Eugenio Martinelli, Dror Seliktar, Sonia Melino(2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt) (Cell Counting Kit 8 (WST-8 /CCK8) (ab228554), Abcam) [30,31], as indicated in the figure legends. After each treatment, the medium was replaced

(9) Development of a novel medium throughput flow-cytometry based micro-neutralisation test for SARS-CoV-2 with applications in clinical vaccine trials and antibody screeningPLOS ONENovember 30, 2023Sophie O’Reilly, Grace Kenny, Tamara Alrawahneh, Nathan Francois, Lili Gu, Matthew Angeliadis, Valentin de Masson d’Autume, Alejandro Garcia Leon, Eoin R. Feeney, Obada Yousif, et al.humidified incubator. After 18h, cell viability was determined using Cell Counting Kit 8 (CCK8) (Abcam #ab228554) according to manufacturer’s instructions. Following a 2-hr incubation period, the absorbance was read

(10) Unsaturated fatty acid alters the immune response in non-small cell lung adenocarcinoma through regulation of HMGB1 traffickingbioRxiv : the preprint server for biologyNovember 11, 2023Cole-Skinner B, Andre NM, Blankenheim Z, Root K, Simmons GE Jr.1000 cells/well in 96-well plates for 24 hours. Subsequently, 10 μl of CCK-8 solution (Abcam, cat no. ab228554) was added to each well, and the plates were incubated at 37 °C for 2 h. Finally, the absorbance was

(11) A Dexamethasone-Loaded Polymeric Electrospun Construct as a Tubular Cardiovascular ImplantPolymers (Basel)November 6, 2023Stavroula Kyriakou, Sergio Acosta, Ikram El Maachi, Stephan Rütten, Stefan JockenhoevelProliferation The proliferation of HUVECs and HUVSMCs was evaluated by using a Cell Counting Kit 8 (WST-8/CCK8) (ab228554, Abcam, UK). The samples used were either fibrin gels molded on top of the polymeric sheets or polymeric

(12) Tumor-suppressive microRNA-152 inhibits the proliferation of Ewing’s sarcoma cells by targeting CDK5R1Scientific ReportsOctober 29, 2023Masanori Kawano, Kazuhiro Tanaka, Ichiro Itonaga, Tatsuya Iwasaki, Yuta Kubota, Hiroshi Tsumuraflow cytometer. Cell counting kit‑8 (CCK‑8) assay Cell viability assay was performed using CCK‑8 kit (Abcam, cat. no. ab228554). Briefly, cells were seeded into 96‑well microtiter plates at a density of 6.0 × 103 cells/well, followed

(13) Metabolic stratification of human breast tumors reveal subtypes of clinical and therapeutic relevanceiScienceOctober 20, 2023Mohammad A. Iqbal, Shumaila Siddiqui, Kirk Smith, Prithvi Singh, Bhupender Kumar, Salem Chouaib, Sriram Chandrasekaranmedia,Gibco,Cat#A1451701 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, CCK8 kit,Abcam,Cat#ab228554 ,, Deposited data,Deposited data,Deposited data ,, Code availability,This paper,https://github.com/kirksmi/BreastCancerClustering METABRIC

(14) Paroxetine protects against bleomycin-induced pulmonary fibrosis by blocking GRK2/Smad3 pathwayAging (Albany NY)October 15, 2023Kaochang Zhao, Hanxiang Nie, Zheng Tang, Guozhong Chen, Jizhen Huangand added into a 96-well plate. Then the cell viability was detected using a commercial assay kit (#ab228554, Abcam) according to the instruction. Data analysis A log-rank test was used to draw survival curves.

(15) Inflammatory cell death induced by 5-aminolevulinic acid-photodynamic therapy initiates anticancer immunityFrontiers in OncologyOctober 2, 2023Lingyan Wang, Vipin Shankar Chelakkot, Nick Newhook, Stephanie Tucker, Kensuke Hirasawacaspase-3 inhibitor (Z-DEVD-FMK) were purchased from R&D systems (Minneapolis, MN). Cell counting Kit (WST-8/CCK8) (ab228554) (Abcam, Boston, MA) was used to determine the cell viability. LDH assay kit (Thermo Fisher Scientific,

(16) Effects of low doses of methylmercury (MeHg) exposure on definitive endoderm cell differentiation in human embryonic stem cellsArchives of ToxicologyOctober 1, 2023Bai Li, Xiaolei Jin, Hing Man Chanviability and morphology Cell viability was determined by a Cell Counting Kit 8 (WST-8/CCK8) (Catalog no. ab228554, Abcam Inc.) according to the manufacturer’s instructions. In brief, hESCs were seeded, induced, and

(17) Low-dose daylight exposure induces nitric oxide release and maintains cell viability in vitroScientific ReportsSeptember 28, 2023Gareth Hazell, Marina Khazova, Paul O’Mahoneyassays Assessment of cell survival after light exposure was performed using a cell counting kit 8 (WST-8, Abcam ab228554). The kit assesses cell survival via the action of a tetrazolium salt that when ingested by live cells

(18) Non-thermal atmospheric pressure plasma treatment increases hydrophilicity and promotes cell growth on titanium alloys in vitroScientific ReportsSeptember 8, 2023Woo-Lam Jo, Young-Wook Lim, Soon-Yong Kwon, Ji-Hoon Bahk, Jungsung Kim, Taejin Shin, YongHwa Kimspecimens and incubated for 4 h, 24 h, 5, 10, or 15 days. All samples were reacted with WST-8 reagent (ab228554; Abcam, UK). Cell proliferation was assessed through absorbance measurements (PowerWave XS microplate

(19) PTBP1 plays an important role in the development of gastric cancerCancer Cell InternationalSeptember 5, 2023Zewen Chu, Miao Zhu, Yuanyuan Luo, Yaqi Hu, Xinyi Feng, Haibo Wang, Masataka Sunagawa, Yanqing Liu(Olympus, Japan), and Western blot. CCK-8 assay Use the Cell Counting Kit 8 (Abcam, UK, Cat. no. ab228554). Plate 3000 cells per well in 96-well plates (Corning, USA, Cat. no. 3599). Add test compounds into

(20) L‐Arginine‐Loaded Gold Nanocages Ameliorate Myocardial Ischemia/Reperfusion Injury by Promoting Nitric Oxide Production and Maintaining Mitochondrial FunctionAdvanced ScienceSeptember 1, 2023Zekun Wang, Nana Yang, Yajun Hou, Yuqing Li, Chenyang Yin, Endong Yang, Huanhuan Cao, Gaofei Hu, Jing Xue, Jialei Yang, et al.added to 96‐well plates, and then, the cells were subjected to OGD/R treatment. A Cell Counting Kit‐8 (ab228554, Abcam) was used to determine cell viability. Briefly, H9C2 cells (1×106 cells well−1) were seeded into

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(1)

4/19/2023Great Kit for Cell Viability and More Seth TompkinsThis kit is marketed as a way to measure cell viability. This is true, but it can do so much more. The assay works by measuring active mitochondria (more mitochondria, the more the dye is reduced). So... Read Review
(1)

10/4/2022Good Cell Viability Assay Kit Yashavantha VishweshwaraiahWe used this kit for cell viability microplate assay using various cell lines and expression conditions. We got consistent and reproducible results.

Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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Cell Counting Kit 8 (WST-8 / CCK8) from Abcam

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